University of Florida Biological Scientist II in Gainesville, Florida
Biological Scientist II
Apply nowJob no:503216
Work type:Staff Full-Time
Location:Main Campus (Gainesville, FL)
Department:29150000 - MD-OPHTHALMOLOGY
Biological Scientist II
Design vector plasmids, primers, strategy for cloning and point mutagenesis. Perform cloning and sequencing analysis. Express proteins of interest in cell cultures and characterize their expression using western blot, microscopy, immunoprecipitation, quantitative PCR and chromatography. Perform florescent and luciferase based assays.
Perform ocular injections on mice. Harvest and prepare ocular tissue for microscopic examination, including dissecting, sectioning and immunostaining. Perform experiments on live mice involving the operation of diagnostic, in-vivo laboratory equipment, including electroretinogram (ERG), digital fundus camera, optical coherence tomography (OCT). Collecting and transforming data obtained from the equipment listed above for research staff, including familiarizing oneself with the computer based data acquisition software for each apparatus.
Assist PI with purchasing equipment, consumables and chemicals for the lab.
Manage mouse breeding colonies, including genotyping, managing breeding strategies, managing production for experiments.
Record and analyze data, prepare data for publication or poster presentation, report findings at lab meetings. Study literature, develop protocols, and learn necessary methods as required.
$35,000 - $45,000
Doctoral degree in biology or a closely related field; or a master's degree in biology or a closely related field and one year of relevant experience; or a bachelor's degree in biology or a closely related field and three years of relevant experience.
The ideal candidate should have working experience expressing proteins in cell culture (HEK293), perform with minimal supervision cloning and point mutagenesis, western blotting, immunoprecipitation, qPCR and imaging. Experience with mouse handling, genotyping and preparation of tissue for immunofluorescent analysis is required. Preference will be given to the applicants who can demonstrate ability to complete experimental projects and have interest in mechanistic studies of pathobiology.
Special Instructions to Applicants:
This is a time limited position.
Please upload a resume, cover letter, and a list of three references in order to be considered.
Application must be submitted by 11:55 p.m. (EDT) of the posting end date.
Health Assessment Required:Yes
Advertised:11 Aug 2017Eastern Daylight Time
Applications close:19 Aug 2017Eastern Daylight Time